nitroblue tetrazolium test…

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what the risks are --> nitroblue tetrazolium test, nbt test, nitroblue tetrazolium test measures the ability of certain cells, in the immune system to convert a colorless chemical, nitroblue tetrazolium, nbt, to a deep blue color, blood is drawn, a vein, the elbow, the hand, puncture site is cleaned, antiseptic, an elastic band, around the upper arm to apply pressure, vein to swell, blood, a needle is inserted, vein, blood is collected in an air-tight vial, a syringe, band is removed, restore circulation, once the blood has been collected, needle is removed, puncture site is covered to stop any bleeding, nbt is added to a sample of white blood cells, neutrophils, white blood cell in immune system that ingest, kill bacteria, make a chemical that kills the bacteria, in chronic granulomatous disease, cgd, chemical is absent, neutrophils, able to ingest bacteria, not kill them, chemical that kills the bacteria, same that turns nbt from clear to deep blue, chemical is absent, nbt is added, sample, not change color, seen by looking, white blood cells under an ordinary microscope, , child, have this test performed, to explain how the test, demonstrate on a doll, explain the reason, knowing the "how, why", reduce the anxiety child feels, the needle is inserted to draw blood, feel moderate pain, or only a prick, stinging sensation, some throbbing, this test is performed, a screen, cgd, sample, neutrophils, it turn blue, nbt is added, indicates that the neutrophils, producing the chemical necessary to kill bacteria, sample, does not change color, nbt is added, neutrophils, missing the chemical necessary to kill bacteria, indicate cgd, risks associated, having blood drawn, slight, excessive bleeding, fainting, feeling light-headed, hematoma, blood accumulating under the skin, infection, a slight risk, the skin is broken, multiple punctures to locate veins.